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reference protein vinculin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc reference protein vinculin
    Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. <t>Vinculin</t> was the loading control for AHR <t>and</t> <t>GAPDH</t> for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.
    Reference Protein Vinculin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference protein vinculin/product/Cell Signaling Technology Inc
    Average 96 stars, based on 171 article reviews
    reference protein vinculin - by Bioz Stars, 2026-04
    96/100 stars

    Images

    1) Product Images from "Reprogramming offspring liver health: maternal indole supplementation as a preventive strategy against MASLD"

    Article Title: Reprogramming offspring liver health: maternal indole supplementation as a preventive strategy against MASLD

    Journal: eBioMedicine

    doi: 10.1016/j.ebiom.2025.106098

    Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. Vinculin was the loading control for AHR and GAPDH for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.
    Figure Legend Snippet: Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. Vinculin was the loading control for AHR and GAPDH for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.

    Techniques Used: Simple Western, Control, Gene Expression, Activation Assay, Isolation



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    reference protein vinculin  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc reference protein vinculin
    Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. <t>Vinculin</t> was the loading control for AHR <t>and</t> <t>GAPDH</t> for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.
    Reference Protein Vinculin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference protein vinculin/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    reference protein vinculin - by Bioz Stars, 2026-04
    96/100 stars
    		  Buy from Supplier
    reference proteins  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc reference proteins
    Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. <t>Vinculin</t> was the loading control for AHR <t>and</t> <t>GAPDH</t> for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.
    Reference Proteins, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference proteins/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    reference proteins - by Bioz Stars, 2026-04
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. Vinculin was the loading control for AHR and GAPDH for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.

    Journal: eBioMedicine

    Article Title: Reprogramming offspring liver health: maternal indole supplementation as a preventive strategy against MASLD

    doi: 10.1016/j.ebiom.2025.106098

    Figure Lengend Snippet: Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. Vinculin was the loading control for AHR and GAPDH for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.

    Article Snippet: Data were normalised to reference protein vinculin (1:1000; CST Cat# 13901, RRID: AB_2728768 ), GAPDH (1:500; CST Cat# 2118, RRID: AB_561053 ), or total protein (with the Total Protein detection module) using Compass software (ProteinSimple).

    Techniques: Simple Western, Control, Gene Expression, Activation Assay, Isolation